细胞动态培养瓶3D培养\ 微重力培养瓶DCF025M 型号: SG-DCF-025M | 价格: 请联系我们详询,谢谢！ 由赛吉生物科技（SAGE-BIO TECH,)设计的新型可重复细胞动态培养瓶FDC ( Flask for Dynamic Culture ) ，适用于100%充满培养液、低剪切力、无气泡的3D动态培养，规格包括标准的25cm2,75cm2以及150cm2等，有效生长表面积分别为25、75以及150平方厘米。与传统的培养瓶相比，该系列新型培养瓶可重复灭菌多次使用，瓶身材料采用无毒、耐腐蚀材料制作，可反复多次耐受121度高温高压灭菌（湿热法），并且能够方便做到100%充满培养液、低剪切力、无气泡培养，适用于几乎所有动态环境培养、3D培养、微重力模拟以及超重力模拟培养等，是细胞由传统静态培养迈向动态培养的又一次革新。 热线电话: +86 0 15995516226、+86 0 13862149980 电子邮箱: info@sage-bio.com、carlos_wei@sage-bio.com 留言咨询收藏 攸寻医疗科技科技欢迎您留言 ... ... 为及时与您取得联系并回复您的关注，请务必输入您的姓名、咨询内容及有效的联系电话等，谢谢！ 仅作联络之用，我司承诺保密，敬请放心填写！ 会员提交咨询后，可登录网站进入“会员中心 - 我的咨询”查看管理员的回复内容；非会员提交的咨询，回复内容不会在前台公开显示。 留言加载中... 在线留言咨询 咨询内容： 联系电话： 电子邮箱： 验证码： 联系人： 已自动填入（可修改） ×

图片展示

DCF-025M拥有双交换膜	25CM2超白玻璃培养板	100%满充培养液约7.5ml

产品概述

      赛吉生物（SAGE-BIO）推出的 SG-DCF 系列可重复细胞动态培养瓶，是传统培养瓶的革命性升级产品，专为 3D 动态培养场景设计，完美适配其自主研发的 DARC-G4.0S/P 通用重力环境模拟系统。该系列提供 SG-DCF025（25cm²）、SG-DCF075（75cm²）、SG-DCF150（150cm²）三种规格，有效生长表面积与型号精准匹配。瓶体采用无毒耐高温材料，可反复耐受 125℃高温高压灭菌，配合可拆解的超白玻璃生长层，实现长期重复使用。核心优势在于能建立 100% 充满培养液、无气泡的低剪切力环境，20mm 以上硅基气体交换膜保障高效气体交换，8mm 大加料口（距生长面仅 3mm）简化取样操作，顶部快速接头可便捷排空气泡。高清玻璃观测窗支持显微镜实时观察，全液层填充设计确保光学观测真实性，适配赛吉重力模拟系统后，可广泛应用于航天医学、组织工程等动态培养研究。气体交换膜与单通阀为一次性耗材，需按规范更换。

       SG-DCF-025M 内置双气体交换膜，且气体交换膜可快速更换。

The SG-DCF series reusable cell dynamic culture flasks, launched by SAGE-BIO, represent a revolutionary upgrade over traditional culture flasks. Specifically designed for 3D dynamic culture scenarios, they are perfectly compatible with the self-developed DARC-G4.0S/P universal gravity environment simulation system. This series offers three specifications: SG-DCF025 (25cm²), SG-DCF075 (75cm²), and SG-DCF150 (150cm²), with the effective growth surface area precisely matching the model designation.

The flask body is constructed from non-toxic, high-temperature-resistant materials and can withstand repeated autoclaving at 125°C. Combined with the detachable ultra-clear glass growth layer, this enables long-term reuse. A core advantage is its ability to establish a low-shear environment, 100% filled with culture medium and free of bubbles. A silicone-based gas exchange membrane exceeding 20mm in diameter ensures highly efficient gas exchange, while an 8mm large feeding port (only 3mm from the growth surface) simplifies sampling operations. The top quick-connect fitting allows for convenient bubble removal.

A high-clarity glass observation window supports real-time microscopic observation, and the fully liquid-filled design ensures the authenticity of optical monitoring. When adapted for use with SAGE's gravity simulation systems, it can be widely applied in dynamic culture research areas such as space medicine and tissue engineering. The gas exchange membrane and one-way valve are single-use consumables and must be replaced according to specifications.

优势介绍

       与传统的普通培养瓶相比，该系列新型培养瓶可重复灭菌多次使用，瓶身材料采用无毒、耐腐蚀材料制作，可反复多次耐受125度高温高压灭菌，并且能够方便做到100%充满培养液、低剪切力、无气泡培养，适用于几乎所有动态环境培养、3D培养等，是传统培养瓶的革新。针对 3D 动态培养场景研发的革新性产品，可直接适配其自主研发的 DARC-G4.0S/P 通用重力环境模拟系统，解决传统培养瓶在动态培养中的核心痛点。搭配 1mm 超薄玻璃材质，光学性能全面优于传统透明塑料培养瓶，这体现在：

1、SG-DCF动态培养瓶的光学优势

1.1 玻璃观测窗透光率达 92%-95%，传统聚苯乙烯（PS）塑料培养瓶透光率为 88%-92%，聚酸酯（PC）塑料瓶透光率为 90%-95%； 

1.2 光学畸变优化，玻璃观测窗采用气悬浮成型工艺，光学畸变指数低至 0.15μrad，透射波前畸变 PV 值≤λ/4@632.8nm（约 0.158μm），较传统塑料瓶（畸变率通常 0.5-1μrad）减少 70%-85% 光学畸变，消除边缘重影与光斑干扰；

1.3 成像真实性提升，结合 1mm 超薄玻璃与全液层填充设计，进光量较塑料瓶提升 15%，色彩还原误差降低 60%，显微镜下细胞细节解析度提升 20%，光学观测真实性较传统塑料瓶综合提升 30%-40%。

Compared to traditional ordinary culture flasks, this new series of flasks can be sterilized and reused multiple times. The flask body is made of non-toxic, corrosion-resistant material that can withstand repeated high-temperature高压 sterilization at 125°C. It easily enables a 100% fluid-filled, low-shear stress, and bubble-free culture environment, making it suitable for almost all dynamic environment cultures and 3D cultures, representing a innovation over traditional flasks. As a groundbreaking product developed for 3D dynamic culture scenarios, it can be directly compatible with their self-developed DARC-G4.0S/P Universal Gravity Environment Simulation System, effectively addressing the core pain points of traditional flasks in dynamic culture.

Paired with 1mm ultra-thin glass, its optical performance is comprehensively superior to traditional transparent plastic culture flasks, as evidenced by:

1. Optical Advantages of the SG-DCF Dynamic Culture Flask

1.1 The light transmittance of the glass observation window reaches 92%-95%, compared to 88%-92% for traditional Polystyrene (PS) plastic flasks and 90%-95% for Polycarbonate (PC) plastic flasks.

1.2 Optimized Optical Distortion: The glass observation window, manufactured using an air flotation molding process, has an optical distortion index as low as 0.15μrad and a transmitted wavefront distortion PV value of ≤ λ/4 @ 632.8nm (approx. 0.158μm). This reduces optical distortion by 70%-85% compared to traditional plastic flasks (typically 0.5-1μrad), effectively eliminating edge ghosting and speckle interference.

1.3 Enhanced Imaging Fidelity: The combination of the 1mm ultra-thin glass and the fully liquid-filled design increases light intake by 15% compared to plastic flasks, reduces color reproduction error by 60%, and improves cellular detail resolution under the microscope by 20%. The overall authenticity of optical observation is comprehensively enhanced by 30%-40% compared to traditional plastic flasks.

2、SG-FDC动态培养瓶气体交换能力

3、SG-FDC动态培养瓶超强低吸附能力

对比维度	SG-DCF 特氟龙瓶身	传统 PS 塑料瓶	传统 PC 塑料瓶	特氟龙相对优势
细胞外泌体吸附率（24h）	≤0.5%（HeLa 细胞来源外泌体）	5%-12%	8%-15%	吸附率降低 89%-96%
特殊蛋白吸附率（IgG/TNF-α）	≤1.0%（1mg/mL 蛋白溶液）	8%-15%	10%-18%	吸附率降低 89%-94%
小分子试剂吸附率（如酶 / 染料）	≤0.3%（0.1mM 荧光素 / 胰蛋白酶）	3%-8%	5%-10%	吸附率降低 93%-97%
吸附稳定性（72h 变化量）	≤0.2%	2%-5%	3%-6%	吸附波动降低 90%-97%
批次间吸附差异率	≤0.1%	1.5%-3%	2%-4%	差异率降低 93%-97%

应用场景

       赛吉生物SG-DCF系列可重复细胞动态培养瓶是一款革命性产品，专为克服传统培养瓶在3D动态培养中的局限而设计。它完美适配其DARC-G4.0S/P通用重力环境模拟系统，能够创建一个100%充满培养液、无气泡且低剪切力的理想培养环境。其核心优势在于卓越的光学性能、高效的气体交换和超强的低吸附能力。该培养瓶采用超薄玻璃观测窗，透光率高，光学畸变极低，相比传统塑料瓶，成像真实性和细胞细节解析度提升超过30%，为实时显微镜观察和高精度成像提供了保障。其大面积的硅基气体交换膜使气体交换效率提升达5-13.9倍，并保持高度稳定性。此外，特氟龙瓶身显著降低了对细胞外泌体、蛋白及试剂的吸附（吸附率降低89%以上），确保了培养环境纯净和实验结果可靠性。SG-DCF系列可高温高压灭菌并重复使用，配合便捷的加样与气泡排除设计，使其成为航天医学、组织工程、药物筛选等前沿动态培养研究的理想工具。

The SAGE-BIO SG-DCF series reusable cell dynamic culture flasks represent a revolutionary upgrade over traditional flasks, specifically designed for 3D dynamic culture scenarios. They are perfectly compatible with SAGE's self-developed DARC-G4.0S/P universal gravity environment simulation systems. Key advantages include the creation of an ideal, 100% fluid-filled, bubble-free, and low-shear stress environment. Its core strengths lie in superior optical performance, highly efficient gas exchange, and exceptional low-adsorption capability.The flask features an ultra-thin glass observation window with high light transmittance and minimal optical distortion. Compared to traditional plastic flasks, it enhances imaging authenticity and cell detail resolution by over 30%, ensuring reliable real-time microscopic observation and high-precision imaging. A large-area silicon-based gas exchange membrane boosts overall gas exchange efficiency by 5-13.9 times with remarkable stability. Furthermore, the Teflon bottle body significantly reduces the adsorption of extracellular vesicles, proteins, and reagents (adsorption rate reduced by over 89%), guaranteeing a pure culture environment and reliable experimental results. Being autoclavable and reusable, coupled with convenient sampling and bubble-removal designs, the SG-DCF series is an ideal tool for cutting-edge dynamic culture research in space medicine, tissue engineering, and drug screening.

主要应用场景（但不限于）：

1、航天医学与微重力/超重力生物学研究 Space Medicine and Microgravity/Hyper-gravity Biology Research，在DARC-G4.0S/P系统中模拟不同重力环境，研究重力变化对细胞形态、功能、基因表达的影响，为宇航员健康保障和地外生命科学研究提供平台。
Simulating various gravitational environments in DARC-G4.0S/P systems to study the effects of gravity changes on cell morphology, function, and gene expression, providing a platform for astronaut health research and extraterrestrial life science.

2、三维组织工程与类器官构建 3D Tissue Engineering and Organoid Construction，
其低剪切力环境和高效气体交换特别适合复杂3D结构（如类器官、组织球）的长期动态培养，促进营养渗透和废物排出，助力再生医学。
Its low-shear environment and efficient gas exchange are ideal for long-term dynamic culture of complex 3D structures (e.g., organoids, spheroids), promoting nutrient penetration and waste removal, advancing regenerative medicine.

3、高内涵药物筛选与毒性测试 High-Content Drug Screening and Toxicity Testing，卓越的光学性能确保在动态培养条件下也能获得高质量、无畸变的细胞图像，适用于自动化、高分辨率的成像分析，提升筛选数据的准确性和可靠性。
Superior optical clarity ensures high-quality, distortion-free cell images even under dynamic conditions, suitable for automated, high-resolution imaging analysis, enhancing the accuracy and reliability of screening data.

4、细胞分泌组学与外泌体研究 Cell Secretomics and Exosome Research，特氟龙材质的超低吸附特性有效防止珍贵样本（如外泌体、细胞因子）的损失，确保分泌组学研究结果的真实性，是外泌体高效采集与分析的理想工具。
The ultra-low adsorption of Teflon material effectively prevents the loss of precious samples (e.g., exosomes, cytokines), ensuring the authenticity of secretomics research findings, making it ideal for efficient exosome collection and analysis.

5、长期动态细胞共培养研究 Long-term Dynamic Cell Co-culture Studies，支持不同类型细胞（如上皮细胞与免疫细胞）在持续动态条件下的共培养，稳定的环境利于研究细胞间相互作用、信号传导及肿瘤微环境等。
Enables co-culture of different cell types (e.g., epithelial and immune cells) under continuous dynamic conditions. The stable environment facilitates the study of cell-cell interactions, signaling, and tumor microenvironments.

常见问答

Q：SG-DCF是贵公司原创产品吗，会不会存在专利权纠纷问题？

A：是的，SG-DCF是苏州赛吉生物原创首发产品，是一种新型动态培养瓶，用于支持在动态环境下开展贴壁培养的新型培养瓶；

Q：SG-DCF与普通培养瓶相比具有什么优势？

A：首先普通培养瓶不适合动态培养，比如3D动态培养、微重力模拟培养或者超重力模拟培养；其次SG-DCF相对于普通培养瓶的主要优势体现在：

1、可以方便地完全充满培养液，实现无气泡100%培养液，消除气泡带来的干扰；

2、更好的气体交换能力；

3、相同生长表面积下更少的培养液消耗，25cm2规格的SG-DCF培养液仅7.5ml（约普通T25培养瓶的1/10）；

4、SG-DCF系列采用超白玻璃作为生长表面，配合圆形玻璃视窗（升级版）提供远优于普通培养瓶的光学通透性和极低的光学畸变，更实时镜下观察和高质量成像；

5、SG-DCF系列可拆解、耐高温、可换膜，能多次重复使用，节约使用成本，更环保；

Q：SG-DCF长时间使用会不会存在培养液泄露问题？

A：不会，所有连接部位均由可靠的密封；

Q：SG-DCF在培养箱内使用中会产生新的气泡吗？

A：会，因为长时间在37的环境下使用，培养室内的极少部分水分会通过气体交换膜被蒸发从而在培养室内产生新的气泡，但通过SG-DCF顶部配置的接口，可以借助一次性注射器方便、快读地补充培养液并排空气泡，这也是什么需要选择SG-DCF动态培养瓶的关键原因之一；

Q：在DARC-G系列微重力、超重模拟培养系统上可以使用SG-DCF动态培养瓶吗，能时使用几只：

A：我们所有开发的耗材几乎都考虑到用户已购买设备的兼容性，SG-DCF通过适配器可以方便地在DARC-G使用，可以同时加载20只以上实现高通量微重力模拟培养；

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