Comparative Effectiveness of a Clinostat and a Slow-Turning Lateral Vessel at Mimicking the Ultrastructural Effects of Microgravity in Plant Cells.
2024-01-10
The object of this research was to determine how effectively the actions of a clinostat and a fluid-filled, slow-turning lateral vessel (STLV) mimic the ultrastructural effects of microgravity in plan
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Simulated weightlessness in the design and exploitation of a NMR-compatible bioreactor
2024-01-10
Mammalian cells cultured in simulated weightlessness take advantage of a favorable environment, experiencing low shear stress and reduced turbulence. NMR spectroscopy allows the on-line noninvasive mo
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Rhinovirus replication in HeLa cells cultured under conditions of simulated microgravity
2023-09-26
Rotating-wall vessels (RWVs) allow for the growth of cells under conditions of simulated microgravity. Information about the replication of viruses in simulated microgravity using RWVs has not been re
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Cell Culture for Three-Dimensional Modeling in Rotating Wall Vessels: An Application of Simulated Microgravity
2023-09-25
High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 × 107 cells/ml. Cells on micro
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Comparison of Different In Vivo Incubation Sites to Produce Tissue-Engineered Small Intestine
2023-07-31
Green fluorescent protein transgenic rat pups (3–5 days) were used as donors of intestinal organoids. Harvested intestine was exposed to enzymatic digestion to release intestinal stem cellcontaining o
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Small spheroids of adipose-derived stem cells with timedependent enhancement of IL-8 and VEGF-A secretion
2023-07-29
Wound-healing factors secreted from mesenchymal stem cells (MSCs) modulate the immuneresponse and facilitate proliferation of neighboring cells. Although in vitro three-dimensional(3D) culture techniq
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Development of a Three-Dimensional Enteric Cell Culture Model for In Vitro Studies of the Intestinal Eukaryotic Parasites Cryptosporidium spp.
2023-07-29
Although in vitro cultivation, be it axenic or in the presence ofhost cells, is feasible with species from all major groups of parasites,there are species of medical and/or veterinary importancewhich
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Three-dimensional culture in a microgravity bioreactor improves the engraftment efficiency of hepatic tissue constructs in mice
2023-07-29
Tissue-engineered liver using primary hepatocytes has been considered a valuable new therapeuticmodality as an alternative to whole organ liver transplantation for different liver diseases. The develo
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Molecular mechanisms underlying the enhanced functions of three-dimensional hepatocyte aggregates
2023-07-29
Three-dimensional (3D) culture of hepatocytes leads to improved and prolonged synthetic andmetabolic functions, but the underlying molecular mechanisms are unknown. In order toinvestigate the role of
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Efficient large-scale generation of functional hepatocytes from mouse embryonic stem cells grown in a rotating bioreactor with exogenous growth factors and hormones
2023-07-29
Embryonic stem (ES) cells are considered a potentially advantageous source of hepatocytes for bothtransplantation and the development of bioartificial livers. However, the efficient large-scale genera
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Three-Dimensional Aggregates of Mesenchymal Stem Cells: Cellular Mechanisms, Biological Properties, and Applications
2023-07-29
Mesenchymal stem cells (MSCs) are primary candidates in cell therapy and tissue engineering and are beingtested in clinical trials for a wide range of diseases. Originally isolated and expanded as pla
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Exosomes derived from umbilical cord mesenchymal stem cells in mechanical environment show improved osteochondral activity via upregulation of LncRNA H19
2020-03-05
Background: Exosomes derived from stem cells have been demonstrated to be good candidates for the treatment ofosteochondral injury. Our previous studies have demonstrated that mechanical stimulation c
