The presence of microgravity conditions deeply affects the human body functions at the systemic, organ and cellular levels. This study aimed to investigate the effects induced by simulated-microgravit

Background: Tissue engineering is a strategy of cartilage regeneration, but scaffolds, required for 3D growth of chondrocytes, are still a problem. Methods: Searching for possibilities to improve scaf

Detachment and the formation of spheroids under microgravity conditions can be observed with various types of intrinsically adherent human cells. In particular, for cancer cells this process mimics me

The first experiments using machines and instruments to manipulate gravity and thus learn about its impact to this force onto livingsystems were performed by Sir Thomas Andrew Knight in 1806, exactly

Microgravity and cosmic rays as found in space are difficult to recreate on earth. However, ground-based models exist to simulate space flight experiments. In the present study, an experimental model

The object of this research was to determine how effectively the actions of a clinostat and a fluid-filled, slow-turning lateral vessel (STLV) mimic the ultrastructural effects of microgravity in plan

Mammalian cells cultured in simulated weightlessness take advantage of a favorable environment, experiencing low shear stress and reduced turbulence. NMR spectroscopy allows the on-line noninvasive mo

Rotating-wall vessels (RWVs) allow for the growth of cells under conditions of simulated microgravity. Information about the replication of viruses in simulated microgravity using RWVs has not been re

High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 × 107 cells/ml. Cells on micro

Green fluorescent protein transgenic rat pups (3–5 days) were used as donors of intestinal organoids. Harvested intestine was exposed to enzymatic digestion to release intestinal stem cellcontaining o

Wound-healing factors secreted from mesenchymal stem cells (MSCs) modulate the immuneresponse and facilitate proliferation of neighboring cells. Although in vitro three-dimensional(3D) culture techniq

Although in vitro cultivation, be it axenic or in the presence ofhost cells, is feasible with species from all major groups of parasites,there are species of medical and/or veterinary importancewhich